This assumes you have downloaded and setup the rabbitmqadmin command line tool.

delete binding can be used to remove a binding between an exchange and a queue. In this example, a binding between exchange001 and queue001 with key001 is removed.

rabbitmqadmin delete binding source="exchange001" destination_type="queue" destination="queue001" properties_key="key001"

If the binding is removed, the following should be returned.

binding deleted